Publication-ready volcano plot

Scatter2D graphs are a fundamental visualization tool used to display the relationship between two numerical variables. Each data point is represented as a dot on a two-dimensional plane, where the x-coordinate and y-coordinate correspond to the values of the two variables. This allows for quick identification of trends, clusters, and outliers in the data. Applications range from scientific data analysis to business intelligence, making them highly versatile. Creating effective Scatter2D graphs involves careful consideration of axis scaling, labeling, and color-coding. Highlighting specific data points or regions can improve understanding. Interactive Scatter2D plots further enhance exploration, allowing users to zoom, pan, and select individual data points for detailed examination. Choosing appropriate visualization tools greatly impacts the ability to draw meaningful insights from data.


Economist GGPlot Excel Paul Tol Black And White Solarized Stata Tableau Wall Street CanvasXpress
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       "title": "Publication-ready volcano plot",
       "subtitle": "Differential expression: 1,000 genes",
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log2FC: {logFC}
-log10 p: {-log-pVal}
Regulation: {Group}
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         }
      }

      // Create the configuration for the graph
      var config = {
       "graphType": "Scatter2D",
       "title": "Publication-ready volcano plot",
       "subtitle": "Differential expression: 1,000 genes",
       "xAxis": [
        "logFC"
       ],
       "yAxis": [
        "-log-pVal"
       ],
       "xAxisTitle": "log2 fold change",
       "yAxisTitle": "-log10 p-value",
       "colorBy": "Group",
       "colorKey": {
        "Group": {
         "Increased": "rgba(197,27,38,0.75)",
         "Decreased": "rgba(33,102,172,0.75)",
         "NoChange": "rgba(150,150,150,0.35)"
        }
       },
       "sizeBy": "FC",
       "sizes": [
        3,
        6,
        9,
        12,
        15
       ],
       "backgroundType": "panel",
       "panelBackgroundColor": "rgb(245,245,245)",
       "xAxisGridMajorColor": "rgb(255,255,255)",
       "yAxisGridMajorColor": "rgb(255,255,255)",
       "legendBox": true,
       "legendBackgroundColor": "rgb(255,255,255)",
       "showDecorations": true,
       "decorations": {
        "line": [
         {
          "color": "rgba(120,120,120,0.8)",
          "width": 1,
          "x": 1
         },
         {
          "color": "rgba(120,120,120,0.8)",
          "width": 1,
          "x": -1
         },
         {
          "color": "rgba(120,120,120,0.8)",
          "width": 1,
          "y": 1.301
         }
        ]
       },
       "hoverTemplate": "Gene: {vars}
log2FC: {logFC}
-log10 p: {-log-pVal}
Regulation: {Group}
Fold change: {FC}", "labelBy": "vars", "labelSelect": [ "AND", [ "y", ">", 2 ], [ "OR", [ "x", "<", -1 ], [ "x", ">", 1 ] ] ], "labelSize": 11, "setMinX": -3, "setMaxX": 2.8, "setMaxY": 4.2 } // Event used to create graph (optional) var events = false // Call the CanvasXpress function to create the graph var cX = new CanvasXpress("canvasId", data, config, events); </script> </body> </html>
library(canvasXpress)
y=read.table("https://www.canvasxpress.org/data/r/cX-scatter2d19-dat.txt", header=TRUE, sep="\t", quote="", row.names=1, fill=TRUE, check.names=FALSE, stringsAsFactors=FALSE)
z=read.table("https://www.canvasxpress.org/data/r/cX-scatter2d19-var.txt", header=TRUE, sep="\t", quote="", row.names=1, fill=TRUE, check.names=FALSE, stringsAsFactors=FALSE)
canvasXpress(
data=y,
varAnnot=z,
backgroundType="panel",
colorBy="Group",
colorKey=list(Group=list(Decreased="rgba(33,102,172,0.75)", Increased="rgba(197,27,38,0.75)", NoChange="rgba(150,150,150,0.35)")),
decorations=list(line=list(list(color="rgba(120,120,120,0.8)", width=1, x=1), list(color="rgba(120,120,120,0.8)", width=1, x=-1), list(color="rgba(120,120,120,0.8)", width=1, y=1.301))),
graphType="Scatter2D",
hoverTemplate="Gene: {vars}
log2FC: {logFC}
-log10 p: {-log-pVal}
Regulation: {Group}
Fold change: {FC}", labelBy="vars", labelSelect=list("AND", list("y", ">", 2), list("OR", list("x", "<", "-1"), list("x", ">", "1"))), labelSize=11, legendBackgroundColor="rgb(255,255,255)", legendBox=TRUE, panelBackgroundColor="rgb(245,245,245)", setMaxX=2.8, setMaxY=4.2, setMinX=-3, showDecorations=TRUE, sizeBy="FC", sizes=list(3, 6, 9, 12, 15), subtitle="Differential expression: 1,000 genes", title="Publication-ready volcano plot", xAxis=list("logFC"), xAxisGridMajorColor="rgb(255,255,255)", xAxisTitle="log2 fold change", yAxis=list("-log-pVal"), yAxisGridMajorColor="rgb(255,255,255)", yAxisTitle="-log10 p-value" )
from canvasxpress.canvas import CanvasXpress
from canvasxpress.plot import show_in_browser

if __name__ == "__main__":
  # Define the chart with its data and configuration
  chart: CanvasXpress = CanvasXpress(
    render_to = "scatter2d19",
    data = {
      "y": {
        "data": [
          [0.94, 1.399, 3.5508],
          [0.923, 1.917, 3.3186],
          [-0.234, -1.15, 2.8226],
          [0.326, -1.329, 2.7428],
          [0.159, -1.05, 2.5841],
          [0.062, -0.896, 2.4041],
          [-0.057, 1.006, 2.1794],
          [0.004, -1.016, 2.0992],
          [-0.23, 0.816, 2.0522],
          [-0.134, 1.174, 2.0317],
          [0.024, -0.648, 2.003],
          [0.298, -0.621, 1.999],
          [-0.246, -0.576, 1.8406],
          [-0.416, 1.327, 1.8405],
          [-0.149, 0.74, 1.8137],
          [0.144, 0.624, 1.7512],
          [-0.134, 0.626, 1.7371],
          [-0.25, -0.873, 1.716],
          [0.109, -0.944, 1.655],
          [0.155, -0.671, 1.6509],
          [0.022, 0.616, 1.633],
          [-0.154, -0.519, 1.5754],
          [0.133, 0.912, 1.5739],
          [0.032, 0.692, 1.5642],
          [0.215, -0.601, 1.5373],
          [-0.052, 0.576, 1.5266],
          [0.233, 0.534, 1.5025],
          [0.103, -0.531, 1.4939],
          [0.277, 0.545, 1.4886],
          [0.654, -2.438, 1.485],
          [-0.114, 0.772, 1.4845],
          [-0.179, -0.741, 1.4601],
          [-0.002, 0.535, 1.4464],
          [-0.222, 0.809, 1.4417],
          [0.113, -0.544, 1.4238],
          [0.15, -0.506, 1.3955],
          [-0.144, -0.562, 1.3811],
          [0.033, -0.458, 1.3751],
          [0.165, -0.659, 1.35],
          [0.096, 0.591, 1.3475],
          [-0.022, 0.481, 1.345],
          [0.025, -0.937, 1.3431],
          [-0.337, 0.475, 1.3338],
          [-0.036, 0.687, 1.3194],
          [0.07, -0.505, 1.3086],
          [0.142, 0.491, 1.3038],
          [0.209, 0.803, 1.2919],
          [-0.085, 0.647, 1.2828],
          [-0.325, -0.721, 1.2789],
          [0.158, -0.477, 1.2772],
          [0.026, 0.56, 1.2702],
          [0.182, -0.441, 1.266],
          [0.039, -0.654, 1.2501],
          [0.146, -0.607, 1.2441],
          [-0.101, 0.411, 1.2361],
          [0.407, 0.811, 1.2338],
          [-0.117, 0.76, 1.2275],
          [0.231, 0.477, 1.2126],
          [0.284, 0.648, 1.1959],
          [-0.011, 0.446, 1.1935],
          [-0.063, 0.52, 1.1886],
          [-0.415, -0.616, 1.1866],
          [-0.16, 0.49, 1.1846],
          [-0.37, 0.453, 1.1759],
          [0.162, 0.436, 1.1721],
          [-0.215, 0.599, 1.168],
          [0.243, -0.502, 1.1633],
          [0.021, 0.423, 1.1593],
          [-0.276, 1.169, 1.1517],
          [0.004, 0.588, 1.1509],
          [0.271, 1.917, 1.1379],
          [0.082, 0.578, 1.136],
          [0.283, 0.556, 1.135],
          [-0.187, -0.562, 1.1033],
          [-0.171, -0.747, 1.0994],
          [-0.012, -0.587, 1.0809],
          [0.275, -0.714, 1.077],
          [0.004, 0.493, 1.0752],
          [0.116, 0.5, 1.0723],
          [-0.025, 0.497, 1.0669],
          [-0.313, -0.887, 1.0642],
          [0.092, -0.546, 1.0612],
          [-0.115, 0.389, 1.0542],
          [0.07, -0.434, 1.0519],
          [0.263, -0.513, 1.0492],
          [0.432, -0.743, 1.0481],
          [0.2, -0.394, 1.0424],
          [0.198, -0.617, 1.039],
          [-0.13, 0.6, 1.0334],
          [0.116, 0.588, 1.0331],
          [0.035, -0.444, 1.0305],
          [0.163, 0.735, 1.0177],
          [0.159, -0.573, 1.0128],
          [-0.029, 0.37, 1.0126],
          [0.01, -0.359, 1.0086],
          [0.036, 0.401, 1.0069],
          [0.44, -0.566, 1.0055],
          [0.551, -0.513, 1.0011],
          [0.043, -0.327, 1.0009],
          [0.054, 0.383, 0.9932],
          [0.217, -0.459, 0.9931],
          [-0.37, -0.569, 0.9842],
          [-0.003, 0.405, 0.9833],
          [-0.258, 0.361, 0.9802],
          [-0.103, 0.379, 0.977],
          [0.36, 0.36, 0.975],
          [-0.095, -0.365, 0.9748],
          [-0.339, 0.508, 0.9725],
          [-0.237, -0.454, 0.9693],
          [-0.021, 0.398, 0.968],
          [0.136, -0.49, 0.9668],
          [-0.172, -0.342, 0.9589],
          [0.099, -0.353, 0.9576],
          [0.084, -0.452, 0.9575],
          [0.064, -0.388, 0.9564],
          [0.049, 0.436, 0.9539],
          [0.124, -0.845, 0.9511],
          [0.215, -0.39, 0.946],
          [-0.218, 0.375, 0.9429],
          [-0.101, -0.516, 0.9397],
          [0.583, 1.182, 0.9385],
          [0.168, -0.485, 0.9366],
          [-0.075, -0.532, 0.9346],
          [0.045, 0.342, 0.9312],
          [0.142, 0.375, 0.9292],
          [0.275, 0.524, 0.9255],
          [-0.068, -0.407, 0.9246],
          [-0.248, -0.657, 0.9214],
          [0.061, 0.397, 0.921],
          [-0.061, 0.486, 0.9205],
          [0.276, 1.082, 0.9184],
          [-0.199, 0.323, 0.9183],
          [0.06, -0.477, 0.9142],
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      }
    },
    config = {
      "graphType": "Scatter2D",
      "title": "Publication-ready volcano plot",
      "subtitle": "Differential expression: 1,000 genes",
      "xAxis": ["logFC"],
      "yAxis": ["-log-pVal"],
      "xAxisTitle": "log2 fold change",
      "yAxisTitle": "-log10 p-value",
      "colorBy": "Group",
      "colorKey": {
        "Group": {
          "Increased": "rgba(197,27,38,0.75)",
          "Decreased": "rgba(33,102,172,0.75)",
          "NoChange": "rgba(150,150,150,0.35)"
        }
      },
      "sizeBy": "FC",
      "sizes": [3, 6, 9, 12, 15],
      "backgroundType": "panel",
      "panelBackgroundColor": "rgb(245,245,245)",
      "xAxisGridMajorColor": "rgb(255,255,255)",
      "yAxisGridMajorColor": "rgb(255,255,255)",
      "legendBox": True,
      "legendBackgroundColor": "rgb(255,255,255)",
      "showDecorations": True,
      "decorations": {
        "line": [
          {
            "color": "rgba(120,120,120,0.8)",
            "width": 1,
            "x": 1
          },
          {
            "color": "rgba(120,120,120,0.8)",
            "width": 1,
            "x": -1
          },
          {
            "color": "rgba(120,120,120,0.8)",
            "width": 1,
            "y": 1.301
          }
        ]
      },
      "hoverTemplate": "Gene: {vars}<br/>log2FC: {logFC}<br/>-log10 p: {-log-pVal}<br/>Regulation: {Group}<br/>Fold change: {FC}",
      "labelBy": "vars",
      "labelSelect": [
        "AND",
        ["y", ">", 2],
        [
          "OR",
          ["x", "<", -1],
          ["x", ">", 1]
        ]
      ],
      "labelSize": 11,
      "setMinX": -3,
      "setMaxX": 2.8,
      "setMaxY": 4.2
    },
    width = 600,
    height = 600
  )

  # Display the chart in its own Web page
  show_in_browser(chart)
Run this notebook: Open in Binder
from canvasxpress.canvas import CanvasXpress
from canvasxpress.plot import graph

graph(
  CanvasXpress(
    render_to = "scatter2d19",
    data = {
      "y": {
        "data": [
          [0.94, 1.399, 3.5508],
          [0.923, 1.917, 3.3186],
          [-0.234, -1.15, 2.8226],
          [0.326, -1.329, 2.7428],
          [0.159, -1.05, 2.5841],
          [0.062, -0.896, 2.4041],
          [-0.057, 1.006, 2.1794],
          [0.004, -1.016, 2.0992],
          [-0.23, 0.816, 2.0522],
          [-0.134, 1.174, 2.0317],
          [0.024, -0.648, 2.003],
          [0.298, -0.621, 1.999],
          [-0.246, -0.576, 1.8406],
          [-0.416, 1.327, 1.8405],
          [-0.149, 0.74, 1.8137],
          [0.144, 0.624, 1.7512],
          [-0.134, 0.626, 1.7371],
          [-0.25, -0.873, 1.716],
          [0.109, -0.944, 1.655],
          [0.155, -0.671, 1.6509],
          [0.022, 0.616, 1.633],
          [-0.154, -0.519, 1.5754],
          [0.133, 0.912, 1.5739],
          [0.032, 0.692, 1.5642],
          [0.215, -0.601, 1.5373],
          [-0.052, 0.576, 1.5266],
          [0.233, 0.534, 1.5025],
          [0.103, -0.531, 1.4939],
          [0.277, 0.545, 1.4886],
          [0.654, -2.438, 1.485],
          [-0.114, 0.772, 1.4845],
          [-0.179, -0.741, 1.4601],
          [-0.002, 0.535, 1.4464],
          [-0.222, 0.809, 1.4417],
          [0.113, -0.544, 1.4238],
          [0.15, -0.506, 1.3955],
          [-0.144, -0.562, 1.3811],
          [0.033, -0.458, 1.3751],
          [0.165, -0.659, 1.35],
          [0.096, 0.591, 1.3475],
          [-0.022, 0.481, 1.345],
          [0.025, -0.937, 1.3431],
          [-0.337, 0.475, 1.3338],
          [-0.036, 0.687, 1.3194],
          [0.07, -0.505, 1.3086],
          [0.142, 0.491, 1.3038],
          [0.209, 0.803, 1.2919],
          [-0.085, 0.647, 1.2828],
          [-0.325, -0.721, 1.2789],
          [0.158, -0.477, 1.2772],
          [0.026, 0.56, 1.2702],
          [0.182, -0.441, 1.266],
          [0.039, -0.654, 1.2501],
          [0.146, -0.607, 1.2441],
          [-0.101, 0.411, 1.2361],
          [0.407, 0.811, 1.2338],
          [-0.117, 0.76, 1.2275],
          [0.231, 0.477, 1.2126],
          [0.284, 0.648, 1.1959],
          [-0.011, 0.446, 1.1935],
          [-0.063, 0.52, 1.1886],
          [-0.415, -0.616, 1.1866],
          [-0.16, 0.49, 1.1846],
          [-0.37, 0.453, 1.1759],
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